Purification and properties of the replicative intermediate of the RNA bacteriophage R17.
نویسنده
چکیده
24 Brimacombe, R., J. Trupin, M. Nirenberg, P. Leder, M. Bernfield, and T. Jaouni, these PROCEEDINGS, 54, 954 (1965). 25 S61, D., E. Ohtsuka, D. S. Jones, R. Lohrmann, H. Hayatsu, S. Nishimura, and H. G. Khorana, these PROCEEDINGS, 54, 1378 (1965). 26Brenner, S., A. 0. W. Stretton, and S. Kaplan, Nature, 206, 994 (1965). 27 Weigert, M. G., and A. Garen, Nature, 206, 992 (1965). 28Ambler, R. P., Biochem. J., 89, 349 (1963). 29 Munkres, K. D., N. H. Giles, and M. E. Case, Arch. Biochem. Biophys., 109, 397 (1965). 30 Munkres, K. D., and F. M. Richards, Arch. Biochem. Biophys., 109, 466 (1965). 31 Woodward, D. O., and K. D. Munkres, these PROCEEDINGS, 55, 872 (1966).
منابع مشابه
Replication of bacteriophage ribonucleic acid: analysis of the ultrastructure of the replicative form and the replicative intermediate of bacteriophage R17.
A detailed qualitative and quantitative comparison was made of the ultrastructure of single-stranded ribonucleic acid (RNA) from bacteriophage R17 and double-stranded replicative form (RF) and replicative intermediate (RI) from cells infected with this bacteriophage. The nucleic acids were prepared for electron microscopy by the protein monolayer spreading technique of Kleinschmidt. Single-stra...
متن کاملReplication of bacteriophage ribonucleic acid: some properties of native and denatured replicative intermediate.
Purified replicative form (RF) and replicative intermediate (RI) prepared from Escherichia coli cells infected with the ribonucleic acid (RNA) bacteriophage R17 were denatured with dimethyl sulfoxide at 37 C or in aqueous solvents of low ionic strength at 97 C. Denaturation was demonstrated for RF and RI by an increase in specific infectivity and a striking change in the hyperchromicity curves ...
متن کاملStructure and function of bacteriophage R17 replicative intermediate ribonucleic acid. II. Properties of the parental labeled molecule.
Replicative intermediate ribonucleic acid (RNA), designated RI, which contained parental RNA labeled with (32)P was separated by filtration through agarose from the nucleic acids prepared from (32)P-labeled RNA phage-infected Escherichia coli. A larger amount of ribonuclease-sensitive parental label was found in the rapidly sedimenting forms of RI than in the slower sedimenting forms, indicatin...
متن کاملDirect genetic selection of two classes of R17/MS2 coat proteins with altered capsid assembly properties and expanded RNA-binding activities.
RNA challenge phages are derivatives of bacteriophage P22 that enable direct genetic selection for a specific RNA-protein interaction. The bacteriophage P22 R17 encodes a wild-type R17 operator site and undergoes lysogenic development following infection of susceptible bacterial strains that express the R17/MS2 coat protein. A P22 R17 derivative with an OcRNA site (P22 R17 [A(-10)U]) develops l...
متن کاملPurification of RNA and RNA-protein complexes by an R17 coat protein affinity method.
We describe an affinity chromatography method to isolate specific RNAs and RNA-protein complexes formed in vivo or in vitro. It exploits the highly selective binding of the coat protein of bacteriophage R17 to a short hairpin in its genomic RNA. RNA containing that hairpin binds to coat protein that has been covalently bound to a solid support. Bound RNA-protein complexes can be eluted with exc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 55 6 شماره
صفحات -
تاریخ انتشار 1966